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Discussion on Meeting the Challenge of Molecule Specific Analysis and Imaging in Organic and Bio-Systems - Thursday

AIM: To provide a forum for deep and extensive discussion of this major developing area for SIMS.

BACKGROUND: The SIMS XV Organising Committee believes that in-depth scientific discussion is very important to derive full benefit from the new work presented at the conference. However, this is very difficult to realise in the structures of our usual meeting. We therefore plan to introduce a full day Discussion after the style of the Faraday Discussions of the Royal Society of Chemistry (see Faraday). The mass spectral capability of ToF-SIMS would seem to make it a powerful tool for the study of complex organic and bio-systems systems. To fully realize this potential however requires a number of major challenges to be met and overcome. Thus the topic area of the Discussion will be "Meeting the Challenge of Molecule Specific Analysis and Imaging in Organic and Bio-Systems".

Discussion Day Programme

Download all papers as zip file here or individually click on the paper codes below.

08.40 to 09.50 Keynote speaker: Professor Andrew Ewing, Penn State University.
DIS-I-Th-A01 Molecule Specific Imaging and Analysis in Biology: What are the Challenges and The Important Applications

09.50 to 11.00 Session 1 – Towards high sensitivity and high spatial resolution
A fundamental discussion on how the challenge of sensitivity and spatial resolution can be met.

DIS-O-Th-A02 Ron Heeren L.A. McDonnell, S. Luxembourg, A.F.M. Altelaar and S.R. Piersma, FOM Institute, Amsterdam – Why don’t biologists use SIMS

DIS-O-Th-A03 Barbara Garrison and Nick Winograd, Penn State University – Improvements in SIMS continue. Is the end in sight?

DIS-O-Th-A04 Emrys Jones, John Fletcher, Nicholas Lockyer and John C. Vickerman, The University of Manchester – ToF-SIMS analysis of bio-systems – Are polyatomic primary ions the solution?

11.20 to 13.00 Session 2 – Data handling – from data overload to analytical clarity
The increased sensitivity that is being achieved results in extremely complex spectra. A discussion on methods to access the information content of SIMS spectra and images and the robustness of the conclusions drawn from them.

DIS-O-Th-A05 David Castner and Daniel Graham, The University of Washington, Seattle, USA – Information from Complexity: Challenges of ToF-SIMS Data Interpretation

DIS-O-Th-A06 Roy Goodacre, John Fletcher, Alex Henderson, Charlotte Thompson, Nicholas Lockyer and John C Vickerman, University of Manchester, UK – Rapid discrimination of the casual agents of urinary tract infection using ToF-SIMS with chemometric cluster analysis

DIS-O-Th-A07 Bonnie Tyler, University of Utah, USA – Multivariate statistical image processing for molecule specific imaging in organic and bio-systems

DIS-O-Th-A08 Joseph Gardella and Tammy M Milillo, University at Buffalo, State University of New York, USA. - Spatial statistics and interpolation methods for ToF-SIMS Imaging

Lunch 13.00 to 14.00

14.00 to 18.00 Session 3 - Localization and quantification of elements and molecules in cell culture, tissue, biosensors and engineered arrays
This is the most challenging area of bio-analysis. It challenges sensitivity and spatial resolution and covers elemental markers using both dynamic and static SIMS as well as molecular detection using ToF-SIMS.

This Session will be divided into 2 or 3 discussion periods taking up to 4 papers at a time. The issues that may be addressed include the use of elemental tracers for molecular detection, the use of molecular ions themselves for analysis, the role of sample preparation techniques in obtaining desired data etc. How each discussion period is configured will await the content of the full papers.

16.00 – 16.20 Tea/Coffee break

DIS-O-Th-A09 Claude Lechene, Harvard Medical School, USA – Quantitative imaging of cells with SIMS- Nanoautography with stable isopte tracers

DIS-O-Th-A10 Riccardo Levi-Setti K.L. Gavrilov, Mary Beth Neilly, R. Strick and P.L. Strissel, Enrico Fermi Institute, University of Chicago, USA - High resolution SIMS imaging of cations in mammalian cell mitosis, and in Drosophila polytene chromosomes

DIS-O-Th-A11 Chris Grovenor, Kat Smart, Mat Kilburn, Ben Shore, Jon Dilworth, Barry Martin, Chris Hawes and Ros Rickaby, University of Oxford, UK – Specimen preparation and calibration for NanoSIMS analysis of biological materials

DIS-O-Th-A12 Jean-Luc Guerquin-Kern, Winfried Roemer, Ludger Johannes, Patricia Duchambon, Danièle Carrez, and Ting-Di Wu, Institut Curie-Recherche, France - Cellular localization of a 15N labelled peptidic vector using Nanosims imaging

DIS-O-Th-A13 Jörg Hoheisel, German Cancer Research Centre - Label and amplification-free genotyping of genomic DNA

DIS-O-Th-A14 Heinrich Arlinghaus, Christian Kriegeskotte, Michael Fartmann, Jörg Möller, Dieter Lipinsky, University of Münster, Germany - Mass Spectrometric Characterization of Elements and Molecules in Cell Culture and Tissue

DIS-O-Th-A15 Steven Boxer Carine Galli Marxer, Mary L Kraft, Simon Fishel – Stanford University; Peter K Weber, Ian D Hutcheon – Lawrence Livermore National Laboratory, USA. Quantitative analysis of biological membrane composition with high lateral resolution

DIS-O-Th-A16 David Miller, R.R. Harbottle, F. Possmayer, N.S. McIntyre and N.O. Petersen, University of Western Ontario, Canada – Imaging lipid distributions in model membranes with selectively deuterated components using Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS)

DIS-O-Th-A17 Peter Sjövall, Björn Johansson and Jukka Lausmaa, Swedish National Testing and Research Institute and Karolinska Institute, Sweden – Localisation of lipids in freeze-dried mouse brain sections by imaging ToF-SIMS

DIS-O-Th-A18 Håkan Nygren, Katrin Börner, Per Malmberg and Birgit Hagenhoff, University of Göteborg, Sweden – Localization of Cholesterol in Rat Cerebellum with Imaging TOF-SIMS. Effect of tissue preparation

SIMS XV 15th Annual Secondary Ion Mass Spectrometry Conference
September 12-16th 2005, Manchester, UK

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